Morphological changes in the fast vs slow fiber profiles of the urethras of diabetic pregnant rats

Submitted: 19 May 2011
Accepted: 11 October 2011
Published: 4 November 2011
Abstract Views: 843
PDF: 490
Publisher's note
All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher.

Authors

Background. This study was undertaken to test the hypothesis that diabetes and pregnancy detrimentally affect the normal function of urethral striated muscles in rats, providing a model for additional studies related to urinary incontinence. The aim of this study was to evaluate morphological alterations in the urethral striated muscles of diabetic pregnant rats. Design and methods. Twenty female Wistar rats were distributed into four experimental groups of five rats as follows: virgin, pregnant, diabetic virgin, and diabetic pregnant. Diabetes was induced using streptozotocin administration (40 mg/kg i.v.). The rats were lethally anesthetized, and the urethra and vagina were extracted as a unit. Cryostat sections (6 µm thick) were cut and stained with hematoxylin-eosin, and immunohistochemical procedures were performed and subjected to morphological and semi quantitative analysis. Results. The urethral striated muscle from the diabetic pregnant rats presented with the following variations: thinning and atrophy, disorganization and disruption associated with the colocalization of fast and slow fibers and a steady decrease in the proportion of fast vs slow fibers. Conclusion. Diabetes and pregnancy impair the urethral striated muscle and alter its fiber type distribution.

Dimensions

Altmetric

PlumX Metrics

Downloads

Download data is not yet available.

Citations

Supporting Agencies

FAPESP (Process Number 2008/00989-4).
Gabriela Marini, Botucatu Medical School, UNESP - Univ Estadual Paulista, São Paulo, Brazil.
Experimental Research Laboratory, Department of Gynecology and Obstetrics
Angélica M. Pascon Barbosa, Botucatu Medical School, UNESP - Univ Estadual Paulista, São Paulo, Brazil.

Experimental Research Laboratory, Department of Gynecology and Obstetrics

Débora C. Damasceno, Botucatu Medical School, UNESP - Univ Estadual Paulista, São Paulo, Brazil.

Experimental Research Laboratory, Department of Gynecology and Obstetrics

Selma M. Michelin Matheus, Botucatu Biosciences Institute, UNESP - Univ Estadual Paulista, São Paulo, Brazil.
Department of Anatomy
Rodrigo de Aquino Castro, São Paulo Federal University (UNIFESP), São Paulo, Brazil.
Department of Gynecology
Manoel João B. Castello Girão, São Paulo Federal University (UNIFESP), São Paulo, Brazil.
Department of Gynecology
Marilza V. Cunha Rudge, Botucatu Medical School, UNESP - Univ Estadual Paulista, São Paulo, Brazil.

Experimental Research Laboratory, Department of Gynecology and Obstetrics

How to Cite

Marini, G., Pascon Barbosa, A. M., Damasceno, D. C., Michelin Matheus, S. M., de Aquino Castro, R., Castello Girão, M. J. B., & Cunha Rudge, M. V. (2011). Morphological changes in the fast vs slow fiber profiles of the urethras of diabetic pregnant rats. Urogynaecologia International Journal, 25(1), e9. https://doi.org/10.4081/uij.2011.e9

Similar Articles

1 2 3 > >> 

You may also start an advanced similarity search for this article.